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Immunoblot analysis showing the activation of Erk1/2, p38 and JNK1/2 in Wt and Btk −/− BMMs. The cells were infected with Lm (LO28, MOI 10) for the indicated time period. Total ERK1/2 protein levels served as a loading control. Data are representative of three independent experiments. Sustained activation of Erk1/2 has not been observed in the other two batches.
