Figure 6. Interaction between PAP248-286 and polyanionic candidate microbicides.

300 µg/ml PAP248-286 was incubated with various polyanions at graded concentration and then the remaining peptides in the supernatants were electrophoresed on 10% native polyacrylamide continuous gels. Gels were either stained with Coomassie Blue (A) or subjected to immunoblotting with anti-PAP248-286 polyclonal antibody (B). The concentrations of polyanions were 0, 300, 150, 75, 37.5, 18.75, 9.375, 4.7 µg/ml (from lane 1 to lane 8), respectively.