Figure 3. Effect of chitooligosaccharide (COS) on the production of leptin by 3T3-L1 pre-adipocytes during adipogenesis.

Mouse 33-L1 pre-adipocytes were induced to differentiate in the absence or presence of chitooligosaccharide and cell culture media was harvested for quantification of leptin production on days 2–4 post-induction of differentiation (A) and days 4–6 post-induction of differentiation (B) Data represents mean ± standard error from three independent replicate experiments. Note the differences in the scale of Y-axis representing the leptin concentration (pg/ml) in the media for different figures.