Table 1. Thermodynamics of binding of cJun analogues to cFos. Columns (from left to right) show i) Tm values from thermal denaturation analysis ii) calculated % helicity for each respective pair calculated from circular dichroism spectra and iii) K_D values calculated from thermal denaturation data.

Peptide complex	Tm °C	% Helical	KD(20) (Thermal) µM	*KD(20) M−1 (ITC) µM	N	ΔG(20) kcal/mol (ITC)	ΔH kcal/mol	#TΔS kcal/mol [ΔH – ΔG]
cFos-cFos	−1	20%	325	N/D	N/D	N/D	N/D	N/D
cFos-JunWCANDI	44	42%	0.45	1.2±0.05	0.8±0.01	−7.9±0.03	−14.8±0.3	−6.9±0.3
cFos-1	55	65%	0.50	0.39±0.03	0.8±0.01	−8.6±0.04	−11.6±0.1	−3.0±0.1
cFos-2	53	39%	0.19	1.44±0.09	1.2±0.01	−7.8±0.04	−7.1±0.1	+0.7±0.1
cFos-8	32	38%	21.5	55±12.45	1.0±0.35	−5.7±0.13	−9.5±0.4	−3.8±0.4
cFos-9	38	47%	9.2	N/A	N/A	N/A	N/A	N/A
cFos-10	49	52%	1.7	5.8±0.78	0.8±0.01	−7.0±0.08	−8.4±0.9	−1.4±0.9
cFos-11	47	55%	5.8	5.4±0.28	0.7±0.01	−7.0±0.03	−7.7±0.2	−0.7±0.2
cFos-12	52	40%	1.2	7.6±0.51	1.0±0.02	−6.8±0.04	−6.1±0.2	0.7±0.2
cFos-17	44	40%	7.8	11.4±0.88	1.0±0.03	−6.6±.0.05	−5.4±0.3	1.2±0.3
cFos-20	48	49%	3.8	11.2±0.82	1.1±0.03	−6.6±0.04	−7.5±0.3	−0.9±0.3
cFos-22	51	40%	1.4	11.9±1.16	1.4±0.04	−6.6±0.06	−5.0±0.2	1.6±0.2
cFos-24	58	52%	7.2	7.25±0.64	1.1±0.03	−6.9±0.05	−8.8±0.4	−1.9±0.4

The remaining three columns give stoichiometry of binding and thermodynamic data calculated from ITC, with TΔS calculated according to the Gibbs Helmholtz equation.

^*data calculated using the midpoint of the transition from thermal denaturation profiles (and fit as temperature as a function of lnK_D, with the fit lnK_D = aT+C where a is the gradient, T is the temperature in Celsius and C is the intercept) and calculated at 20°C.

^#Calculated according to TΔS = ΔH−ΔG.