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. 2013 Mar 27;8(3):e59428. doi: 10.1371/journal.pone.0059428

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© 2013 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Purified RpfG_AA-GYP completely lost its PDE activity detected by colorimetric assays. (B–D) RpfG_AA-GYP lost the ability to restore EPS production (B), biofilm formation (C) and virulence to rice (D) of ΔrpfG to the wild-type level. WT: wild-type, EV: empty vector.