|
E. coli
|
|
|
| DH5α |
High efficiency transformation |
|
| pRK600 |
Helper strain in tri-parental mating |
|
|
X. oryzae
|
|
|
| RS105 |
Wild-type, RifR
|
|
|
ΔhgdA
|
In frame deletion of XOC_1984, RifR
|
This Study |
|
ΔrpfG
|
In frame deletion of rpfG, RifR
|
This Study |
|
ΔhgdC
|
In frame deletion of XOC_4564, RifR
|
This Study |
|
ΔhgdA/rpfG
|
In frame deletion of XOC_1984 and rpfG, RifR
|
This Study |
|
ΔhgdA/hgdC
|
In frame deletion of XOC_1984 and XOC_4564, RifR
|
This Study |
|
ΔrpfG/hgdC
|
In frame deletion of rpfG and XOC_4564, RifR
|
This Study |
|
ΔhgdA/rpfG/hgdC
|
In frame deletion of XOC_1984, rpfG and XOC_4564, RifR
|
This Study |
|
ΔgumD
|
In frame deletion of gumD, RifR
|
This Study |
|
ΔpgaA
|
In frame deletion of XOC_0767, RifR
|
This Study |
|
ΔpgaC
|
In frame deletion of XOC_0765, RifR
|
This Study |
|
ΔxagA
|
In frame deletion of XOC_3785, RifR
|
This Study |
|
ΔxagB
|
In frame deletion of XOC_3784, RifR
|
This Study |
|
ΔrpfG/gumD
|
In frame deletion of rpfG and gumD, RifR
|
This Study |
|
ΔrpfG/pgaA
|
In frame deletion of rpfG and XOC_0767, RifR
|
This Study |
|
ΔrpfG/pgaC
|
In frame deletion of rpfG and XOC_0765, RifR
|
This Study |
|
ΔrpfG/xagA
|
In frame deletion of rpfG and XOC_3785, RifR
|
This Study |
|
ΔrpfG/xagB
|
In frame deletion of rpfG and XOC_3784, RifR
|
This Study |
|
Plasmids
|
|
|
| pUFR80 |
Suicide vector for homologous recombination, KmR
|
[60]
|
| pVSP61 |
Expression vector, KmR
|
[61]
|
| pMD18-T |
High efficiency cloning vector, AmpR
|
Takara |
| pVSP61-rpfGxoc
|
Complementation, rpfGxoc cloned in pVSP61, KmR
|
This study |
| pVSP61-rpfFxoc
|
Complementation, rpfFxoc cloned in pVSP61, KmR
|
This study |
| pVSP61-xagAxoc
|
Complementation, xagAxoc cloned in pVSP61, KmR
|
This study |
| pQE30 |
In-vitro expression vector, AmpR
|
This study |
| pQE30-rpfG
|
rpfG cloned in pQE30 for RpfG purification, AmpR
|
This study |
