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. 2013 Mar 27;8(3):e58848. doi: 10.1371/journal.pone.0058848

Figure 7. ERK1/2 inhibitor U0126 and PPARγ inhibitor BADGE blocked the effects of curcumin on TGF-β1-induced EMT in HK-2 cells.

Figure 7

(A) Effects of U0126, BADGE on the expression of α-SMA, PAI-1, E-cadherin, TβR I and TβR II with or without TGF-β1. HK-2 cells were pretreated with curcumin (10 μM) for 12 hours followed by treatment with U0126 (10 μM) or BADGE (10 μM) for 36 hours or HK-2 cells were pretreated with U0126 (10 μM) or BADGE (10 μM) for 1 hour before addition of TGF-β1 (2.5 ng/ml) for 36 hours. (B) Effects of curcmin on the expression of α-SMA, PAI-1, E-cadherin, TβR I and TβR II with TGF-β1 and U0126 or BADGE. HK-2 cells were pretreated with curcumin (10 μM) for 12 hours at indicated dose followed by treatment with U0126 (10 μM) or BADGE (10 μM) for 1 hour before addition of TGF-β1 (2.5 ng/ml) for 36 hours.