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. 2013 Mar 27;8(3):e59630. doi: 10.1371/journal.pone.0059630

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© 2013 Billich et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mice were treated on 5 consecutive days with 40 mg/kg tamoxifen p.o. A, B, Two weeks after tamoxifen treatment of Sgpl1^Flox/Flox Cre^+/− mice (open bars) and of Sgpl1^Flox/Flox Cre^−/− controls (closed bars) (n = 5/group), differential blood cell counts were determined (A) and FACS analysis of lymphocyte subsets was performed (B). C, Time course of blood T cell number reduction in induced Sgpl1^Flox/Flox Cre^+/− mice. (n = 5/group). Blood samples were analysed at the indicated time points by FACS with staining for CD3/CD4 (closed bars) and CD3/CD8-double positive T cells (open bars).