Figure 6. Hepatic nuclear HDAC3 protein content immunoprecipitated (IP) with HDAC4/5/7 antibody (A), representative western blot of cytosolic phospho-HDAC4,5, and 7 (B), cytosolic level of phospho-HDAC4 (C) and phospho-HDAC5/7 (D), and nuclear content of acetyl-foxo1 protein (E) in 4 month-old male rat offspring exposed to ethanol (EtOH) or water only during early, mid, or late pregnancy.

Control and pair-fed offspring were from dams with free access to chow and dams fed the amount of chow consumed by EtOH dams, respectively. A subgroup of EtOH rats was given TUDCA (T) 3 weeks before the experiments. Data shown as the mean±SE, n = 6. *P<0.05, **P<0.01 EtOH vs. control; ^#P<0.05, ^##P<0.01 EtOH vs. pair-fed.