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. 2013 Mar 27;8(3):e60177. doi: 10.1371/journal.pone.0060177

Figure 1. Detection of WT1 protein in urinary exosomes of diabetic patients with or without proteinuria.

Figure 1

A) Representative immunoblots for WT1 and TSG101 proteins in urinary exosome samples from, type 1 diabetic patients with or without proteinuria and healthy controls. Exosomal protein obtained from same urine volume was loaded for all the samples. B) Frequency of WT1 expression in urinary exosomes from diabetic patients with or without proteinuria and healthy controls. All subjects were positive for TSG101 protein, an exosomal marker (data not shown). Densitometry analysis of WT1 bands in: C) Type-1 diabetic patients, using Mann-Whitney U test, and D) Proteinuria and Non-Proteinuria groups, using ANOVA rank test. The boxes indicate median and 25th and 75th percentiles; Outliers are indicated by closed dots. p<0.05 was considered significant.