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. 2013 Mar 27;8(3):e59622. doi: 10.1371/journal.pone.0059622

Figure 1. Characterisation of MCK-tTA-hYAP1 S127A mouse model.

Figure 1

A) Schematic outline of the inducible ‘Tet-Off’ transgenic system. B) Genotyping of MCK-tTA and TRE-hYAP1 S127A alleles in single and double transgenic mice. C) Yap protein levels in skeletal muscles of transgenic mice of indicated genotypes following 25 days with (+) or without (−) doxycycline (dox). Tibialis anterior (TA), gastrocnemius (Gas) and extensor digitorum longus (EDL). D) Muscle specific expression of hYAP mRNA in MCK-tTA-hYAP1 S127A mice 25 days after doxycycline withdrawal. E) Time course of Yap protein expression in tibialis anterior of MCK-tTA-hYAP1 S127A mice following doxycycline removal.