Table 2. List of primers and probes used for real-time RT-PCR.
Gene name | Sequence (5′→3′) | Tm(°C) | Amplicon length |
Tp18SrRNAf | TCTTAGTTGGTGGAGTGATTTGTC | 58.0 | 140 bp |
Tp18SrRNAr | CGCCATCTTCCTTCATCTTGTA | 59.6 | |
TpPHOf | TCTTCTATGCGGATGCCTGAG | 60.4 | 157 bp |
TpPHOr | GCCGTGGCACATTGTTCTG | 60.2 |