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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: J Bone Miner Res. 2013 May;28(5):1101–1116. doi: 10.1002/jbmr.1839

Figure 1. Expression of CYP3A4, CYP24A1, CYP27A1, CYP27B1, CYP2R1, VDR, and PXR genes in human hepatocytes.

Figure 1

Human hepatocytes from three different donors were treated with 10 μM rifampin (RIF), 400 μM phenobarbital (PB), 0.5 μM hyperforin (HF), 50 μM carbamazepine (CBZ), 200 μM levitiracetam (LEV) or vehicle control (CT, 0.1% v/v) for 48 hr, as indicated. Total RNA from each sample was isolated and the expression of CYP3A4, CYP27A1, CYP27B1, CYP24A1, CYP2R1, VDR, and PXR was determined by qRT-PCR assay. Data represent mean ± S.E. (from four replicate determinations of three different liver donors) of the fold-induction in treated cells, compared to those of vehicle treated cells, after normalization to the 18s ribosomal RNA level. *: p < 0.05 for the inductive effect of PXR agonists, compared to corresponding control group.