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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Psychoneuroendocrinology. 2012 Dec 4;38(7):1158–1169. doi: 10.1016/j.psyneuen.2012.11.004

Figure 2. Effect of acute footshock on IL-6 protein, STAT3 phosphorylation and SOCS3 mRNA levels in the hypothalamus.

Figure 2

Rats were sacrificed immediately after 90 min of footshock (0h) or at 0.5h, 3.5h and 24h of recovery. The hypothalami were rapidly dissected and one half used for determination of IL-6 protein levels (A, n=4-6) and STAT3 phosphorylation (P-STAT3, n=4-6) (B) with western blot; the other half of the hypothalamus was used to determine SOCS3 mRNA levels by qPCR (C, n= 10-14). No FS= no footshock. *significantly different from No FS control by Newman-Keuls post-hoc test at p<0.05.