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Immunocytochemical analysis for the expression of cardiac marker protein actinin at 60 × magnification on the tissue culture plate (A, D, G), collagen nanofibers (B, E, H) and poly (glycerol sebacate)/collagen core/shell fibers (C, F, I) comprising of cardiomyocytes (A-C), mesenchymal stem cells-cardiomyocytes co-culture group (D-F) and mesenchymal stem cells (G-I). Nucleus stained with 4,6-diamidino-2-phenylindole hydrochloride.
