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Primers used for amplifying M and ORF3 genes of PEDV
| Name of primer | Forward/reverse | Primer sequence (5′–3′) | Target gene | Fragment size (bp) | Genomic co-ordinates |
|---|---|---|---|---|---|
| M-F | Forward | TACATGCGAATTGACCCCCT | M | 873 | CV777strain (AF353511) |
| M-R | Reverse | ATCCTTGTTAGTGGGTACAGCG | |||
| O-F | Forward | CGTGGTGGGTTTGGTTGAT | ORF3 | 964 | |
| O-R | Reverse | CGGTGACAAGTGAAGCACAGAT |
