Fig. 2.

Interaction between NS1 and NOLC1 as determined by immunoprecipitation. pEGFP-N1-NS1 and pCMV-flag-NOLC1 were co-transfected into the 293T cells, and the cell lysate were subjected to immunoprecipitation with anti-FLAG polyclonal antibody. The immunoprecipitated proteins were detected by western blot with anti-GFP antibody. Lane 1, NS1 as detected by western blot with anti-GFP antibody; lane 2 and lane 3, IP performed with the appropriate transfection as indicated followed by western blot. Beta-actin was detected from whole cell lysate