Fig. 5.

Interaction between domain of NS1 and NOLC1. pEGFP-N1-NS1-RBD and pEGFP-N1-NS1-ED, which express the N-terminal RNA binding domain and C-terminal effector domain of NS1, respectively, were coexpressed with NOLC1 in 293T cells. The protein–protein interactions between truncated NS1 proteins and NOLC1 were investigated by coimmunoprecipitation followed by western blot. a RBD domain plus NOLC1. Lane 1, NOLC1and RBD as determined by IP and western blot; lane 2, Input of RBD (western blot only); lane 3, GFP and RBD as determined by IP and western blot. b ED domain plus NOLC1. Lane 1, NOLC1 and ED as determined by IP and western blot; lane 2, Input of ED (western blot only); lane 3, GFP and ED as determined by IP and western blot. Beta-actin was detected using a whole cell lysate