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. 2013 Mar 20;222(4):437–450. doi: 10.1111/joa.12032

Table 1.

Primers used for RT-PCR and qPCR analysis

Gene Forward primer sequence Reverse primer sequence Amplicon size (bp) GenBank accession no
RT-PCR
 CD29 5′-CAAGAAGGAATGCCTACGTC-3′ 5′-CAATGCCACCAAGTTTCCCAT-3′ 720 FCU27351
 CD166 5′-GCTCCCCAGTATTTATTGCCTTC-3′ 5′-GTAGCACCT TTCCATTCCTGTA-3′ 345 Y13243
 CD45 5′-AGGTAGTAGATGTTTTCCAAGTAGTGA-3′ 5′-ACTTGTCCATTCTGGGCAGGGTAG-3′ 130 XM_002717662
 CD34 5′-AGAACTTTCCAGCATGTTCCAGTTTATG-3′ 5′-GGCTTGCCACATCTTGCTCGGTGA-3′ 95 XM_002717543
qPCR
Col-I 5′-CTGACTGGAAGAGCGGAGAG-3′ 5′-TCTGGGCAATGCTGGGCTGTGTGGG-3′ 129 AY633663 (Tan et al. 2012)
Scx 5′-CAG CGG CAC ACG GCG AAC -3′ 5′-CGT TGC CCA GGT GCG AGA TG -3′ 165 BK000280 (Kuo & Tuan, 2008)
Ppar–γ 5′-AGCAAAGAAGTCGCCATCC-3′ 5′-CGTTCAAGTCAAGGCTCACA-3′ 118 NM_001082148
Runx2 5′-TCAGGCATGTCCCTCGGTAT-3′ 5′-TGGCAGGTAGGTATGGTAGTGG-3′ 54 AY598934
Sox9 5′-AGAGCGAAGAGGACAAGTTCCCCGT-3′ 5′-ATGGGCACCAGCGTCCAGTCGTAG C-3′ 85 XM_002719499
RT-PCR and qPCR
 GAPDH 5′-AACATCATCCCTGCCTCTACTG-3′ 5′-CTCCGACGCCTGCTTCAC-3′ 196 NM_002046 (Kuo & Tuan, 2008)