Table I.
A Selection of Crystallin Protein Structures and Their Homologs
| Crystallina | Assembly | Method | PDB | Species and location |
|---|---|---|---|---|
| Hsp20/alpha-crystallin family | ||||
| α-crystallin (A 1–173 and B 1–175) | ∼800 KDa polymer | UC | – | Homo sapiens lens |
| αB-crystallin assembly (1–175) | 10–40 mer | NanoMS | – | Homo sapiens lens and muscle |
| αB ACD (67–157) | Dimer | X-ray | 2wj7 | Homo sapiens |
| αB R120G ACD (67–157) | Dimer | X-ray | 2y1z | Homo sapiens |
| αB ACD + C-term (68–162) | Runaway dimer | X-ray | 3l1g | Homo sapiens |
| αA ACD + C-term (62–163) | Runaway dimer | X-ray | 3l1f | Bos taurus |
| αB-crystallin assembly (1–175) | ∼500 KDa | SS NMR | 2klr | Homo sapiens lens and muscle |
| αB-crystallin assembly (1–175) | ∼500 KDa | CryoEM | 2ygd | Homo sapiens lens and muscle |
| Hsp20 (HSPB6) (65–162) | Dimer | X-ray | 2wj5 | Rattus norvegicus muscle |
| Hsp27 (HSPB1) (90–171) | – | X-ray | 3q9p | Homo sapiens muscle and neural |
| Tsp 36 (2–313) 2 ACDs per chain | Dimer | X-ray | 2bol | Taenia saginata oncosphere |
| Hsp 16.9 (3–151 and 45–151) | Six dimers | X-ray | 1gme | Triticum aestivum |
| Hsp 16.5 (33–147) archaea | 24 mer | X-ray | 1shs | Methanococcus jannaschii |
| Hsp 16.5 plus engineered insert | 48 mer | X-ray | 4eld | Methanococcus jannaschii |
| Hsp 14.0 (17–123) archaea | 24 mer | X-ray | 3vqk | Sulfolobus tokodaii |
| βγ-crystallin-like domains | ||||
| Truncated βB1-crystallin (42–251) | Dimer | X-ray | 1oki | Homo sapiens lens |
| βB2-crystallin (2–205) | Dimer and tetramer | X-ray | 2bb2 and 1ytq | Bos taurus and Homo sapiens lens |
| βB2-crystallin (15–103) | Dimer of N-domains | X-ray | 1e7n | Mus musculus |
| βB3-crystallin (23–199) | – | X-ray | 3qk3 | Homo sapiens lens |
| βA4-crystallin (8–196) | – | X-ray | 3lwk | Homo sapiens lens |
| γB-crystallin (2–175) | 2-Domain monomer | X-ray | 1amm and 2jdf | Bos taurus and Homo sapiens lens |
| γB-crystallin (88–175) | C-Domain monomer | X-ray | 1dsl | Bos Taurus |
| γB-crystallin (88–173) | Dimer of C-domains | X-ray | 1gam | Bos Taurus |
| γC-crystallin (2–174) | 2-Domain monomer | X-ray | 2vtu | Mus musculus lens |
| γD-crystallin (2–174) | 2-Domain monomer | X-ray | 1hk0 | Homo sapiens lens |
| γE-crystallin (2–174) | 2-Domain monomer | X-ray | 1m8u and 1a5d | Bos taurus and Rattus norvegicus lens |
| γS-crystallin (2–178) | 2-Domain monomer | NMR | 1zwm and 2a5m | Mus musculus lens |
| γS-crystallin (91–177) | Dimer of C-domains | X-ray | 1ha4 | Homo sapiens |
| Urochordate βγ-crystallin (2–83) | 1-Domain monomer | X-ray | 2bv2 | Ciona intestinalis otolith and palp |
| Aim1 g1 (1022–1118) | Domain-1 monomer | X-ray | 3cw3 | Homo sapiens epithelial cells |
| Aim1 g5 (1416–1495) | Domain-5 monomer | NMR | 2dad | Homo sapiens epithelial cells |
| Slime mold Spherulin 3A (2–101) | 1-Domain dimer | X-ray | 1hdf | Physarum polycephalum encystment |
| Archaeal βγ-crystallin (37–120) | 1-Domain monomer | X-ray | 3hz2 | Methanosarcina acetivorans |
| Bacterial Protein S (2–173) | 2-Domain monomer | NMR | 1prr | Myxococcus xanthus spore coat |
| Bacterial Protein S (2–89) | N-Domain monomer | X-ray | 1nps | Myxococcus xanthus spore coat |
| Bacterial βγ-domain (119–206) | 1-Domain monomer | X-ray | 3i9h | Clostridium beijerinckii |
| Bacterial βγ-domain (501–590) | 1-Domain monomer | X-ray | 3hzb | Flavobacterium johnsoniae |
a
Protein sequence numbers are based on the Uniprot entry which includes N-terminal Met. CryoEM, Cryo-electron microscopy; NanoMS, Nano-electrospray Mass Spectrometry; SS NMR, Solid State Nuclear Magnetic Resonance Spectroscopy; UC, Ultracentrifugation.