Skip to main content
. 2013 Jan 24;22(4):434–443. doi: 10.1002/pro.2224

Figure 3.

Figure 3

Extraction and solubilization of OmpF-RAMP1 as a function of surfactant type. (A) Induced cultures were lysed, centrifuged and the insoluble fraction mixed with a test detergent solution at 1% (w/v) concentration. After incubation, the soluble fraction was collected by centrifugation and analyzed by SDS-PAGE. OmpF-RAMP1 was highly soluble in FC15, FC14, and FC13 and moderately soluble in FC12 and FC11 (WC: whole cell lysate, FC15: FOS-CHOLINE-15, FC14: FOS-CHOLINE-14, FC13: FOS-CHOLINE-13, FC12: FOS-CHOLINE-12, FC11: FOS-CHOLINE-11, FC10: FOS-CHOLINE-10, FC9: FOS-CHOLINE-9, and FC8: FOS-CHOLINE-8). (B) OmpF-RAMP1 was solubilized in different concentration of FC15, and the minimum FC15 concentration necessary to solubilize OmpF-RAMP1 is 1% w/v. Percent solubilization of RAMP1 by a given test detergent is reported in terms of the relative ratio of RAMP1 present in the whole-cell extract versus that in a given test detergent solution.