Figure 3.

Similarities in the crystal packings of DDM solubilized EcMscS (a) and HpMscS (b). The surface representation with a highlighted central channel indicates possible lattice contacts between the cytoplasmic domains, with more limited contact between the N-terminal and C-terminal domains. The crystallographic b axis is horizontal, with the sevenfold axis of the central MscS heptamer (along diagonal of the ac plane) in the vertical direction. (c) Crystal packing in the ac plane of HpMscS, with a single channel at the center highlighted with multicolored chains. The red boxes identify the herringbone pattern from each stacking subunit. The red arrow denotes the orientation of each channel, with the head corresponding to the C-terminal, cytoplasmic domain while the tail end denotes the N-terminal, transmembrane domains. The x, y, and z axes indicate the orientation of the structure along the crystallographic a, b, and c axes, respectively. A similar packing arrangement is also present in EcMscS. This figure was created with PyMol.³⁹