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. 2013 Mar 28;4:35. doi: 10.3389/fphar.2013.00035

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Copyright © Pitts, Kulikowski, Tan, Murray, Arcaroli, Tentler, Spreafico, Selby, Kachaeva, McPhillips, Britt, Bradshaw-Pierce, Messersmith, Varella-Garcia and Gail Eckhardt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any thirdparty graphics etc.

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Effects of PF-3758309 on anchorage-independent growth, spheroid formation and migration. (A) HCT116 and GEO were plated in a soft agar assay untreated or treated with 15 nmol/L of PF-3758309 for 14 days. (B) HCT116 and GEO were plated in 3D culture in matrigel to observe spheroid formation. (C) Quantification of spheroid formation of two sensitive and two resistant cell lines. The average spheroids from three separate experiments are displayed. Data is presented as mean ± SEM (t-test: *p <0.001). (D) RKO and SW480 were evaluated for their migratory phenotype in the presence of PF-3758309. Cells grown to 80% confluence were cleared with a scratch and migration was measured by the ability of cells to migrate into scratch after 48 h.