Table 2.

Summary table showing membrane-associated proteins and their predicted or tested localization and bundle sheath (BS) or mesophyll (M) association.

Protein name	Localization (GFP/YFP or pred.)	No. Of TM regions (Octopus)	Spectral count ratio (BS/M)	BS/M ratio determined by RT-PCR
5TM	YFP	8	0.79	0.58 ± 0.11
ER-AP	GFP	3	0.70	0.65 ± 0.02
Hyp2	unknown	11	0.57	0.70 ± 0.27
Hyp3	Cp-pred	4	BS	0.87 ± 0.25
HypE	Cp-pred	2	0.80	0.78 ± 0.14
HypF	Cp-pred	0	0.57	0.65 ± 0.23
HypFD	Cp-pred	4	0.31	0.28 ± 0.15
Mep1	Cp-GFP	12	1.09	1.12 ± 0.56
Mep3	Cp-YFP	4	0.69	3.00 ± 1.56
UP-a	Cp-YFP	1	1.41	0.66 ± 0.11
UP-d	Cp-YFP	4	1.05	0.40 ± 0.05
UP-f	CP-pred	1	3.09	0.84 ± 0.09
PIC/TIC	Cp-YFP	4	1.20	0.99 ± 0.16
PEPC	Cyt	0	0.71	0.30 ± 0.20
NADP-ME	Cp	2	5.32	4.44 ± 1.57

Localization prediction was based on computer prediction or on actual GFP/YFP labeling (Figure 4). The spectral count ratio was taken from the proteomics data (Table S2 in Supplementary Material; Figure 2); gene expression ratios were obtained by RT-PCR (Figure 3). The abbreviations for proteins are identical to those used in both supplementary tables. Spectral count ratios are based on the average of three experiments; RT-PCR-based ratios are mean and standard error of three experiments.