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. 2013 Mar 28;3:1565. doi: 10.1038/srep01565

Figure 3. Relaxation kinetics of tube restoring force after a 10 μm step elongation in the presence or absence of FBAR.

Figure 3

The elongation is performed at 10 μm/s and the position of the particle is recorded with 45 μs time resolution. (a) Force versus time relaxation behavior of the force ΔF shown for three different concentrations of FBAR. Prior to elongation of the tether the force, F, equals the equilibrium holding force, Fh, of the short tether and ΔF = F−Fh = 0. After elongation ΔF at different protein concentrations relaxes back to zero. Inset, images of GUV and tube before and after elongation (images are contrast enhanced for visibility). (b) Superimposed average relaxation behavior of a number of experiments. Experiments are performed at 500 nM (magenta, average of 8 experiments), 50 nM (red curve, average of 6 experiments) and in absence of FBAR (blue curve, average of 8 experiments). The exponential relaxation time, τ, increases with FBAR concentration with τ0 nM = 0.52 ± 0.01 s, τ50 nM = 1.20 ± 0.02 s, τ500 nM = 1.71 ± 0.01 s.