Table 1.
Primers used for real-time PCR
| Gene (abbreviation) | GenBank accession no. | Sequence (5' → 3') | Length of DNA product (bp) |
|---|---|---|---|
|
β-actin (ACTB) |
NM_007393 |
F: CTGTCCCTGTATGCCTCTG |
218 |
| |
|
R: ATGTCACGCACGATTTCC |
|
|
Nuclear factor erythroid 2-related factor 2 (Nrf2) |
NM_010902 |
F: CAGTGCTCCTATGCGTGAA |
109 |
| |
|
R: GCGGCTTGAATGTTTGTC |
|
|
Heme oxygenase 1 (HO-1) |
NM_010442 |
F: ACAGATGGCGTCACTTCG |
128 |
| |
|
R: TGAGGACCCACTGGAGGA |
|
|
NAD(P)H: quinine oxidoreductase 1 (NQO1) |
NM_008706 |
F: CTTTAGGGTCGTCTTGGC |
102 |
| |
|
R: CAATCAGGGCTCTTCTCG |
|
|
γ-Glutamylcysteine synthetase (GCLC) |
NM_010295 |
F: GGATGATGCCAACGAGTC |
180 |
| R: GTGAGCAGTACCACGAATA |