Figure 4. Effects of RNAi-mediated knockdown of TcMet and TcSRC on the JH-dependent induction of TcKr-h1 in Tc81 cells.

Cells were soaked in 50 ng/μL dsRNA for MalE, TcMet, or TcSRC for 60 h. Control cells (−) were not treated with dsRNA. Cells were then incubated in media containing 10 μM JHA (JHA+) or JHA-free media (JHA-) for 2 h, and TcMet (A), TcSRC (B), and TcKr-h1 (C) transcript levels were determined by qPCR (mean ± SD, n = 3). Data were analyzed using Student's t-tests (***P < 0.001; **P < 0.01; *P < 0.05; not indicated, P > 0.05).