Table 3.

Primer sequences, melting temperatures and product sizes for twelve immunogenes in the qPCR analysis

Gene symbol	Primer sequence (5'-3')	Target size (bp)	Tm (°C)
ADAM17	Sense: TGGCAGACAACATCGTGGG	106	59
	Anti-sense: GGGCTTGATGATGCGAACG
ARG2	Sense: GCATTCCGCCCCGAAG	233	65
	Anti-sense:CCACTGAGCGAGGATTCACT
C1R	Sense: TGTGGACCTGGATGAATGT	101	59
	Anti-sense: AATAGCCGCCGATGTAGTT
CFB	Sense: CATTCATGATATTCGGGACCT	92	59
	Anti-sense: GCCCAACCCCAAACACGTA
CD1D	Sense: GTGGCTCCTCTACGACATCT	88	55
	Anti-sense: TTCAGGCTTCACTTGCTTCTC
BCL6	Sense: TTGTCATAGTGGTGAGCCGAGAG	168	65
	Anti-sense: TGAAGTCCAGGAGGATGCAGAAC
ADM	Sense: CGCTACCGCCAGAGTATGAACA	121	59
	Anti-sense: CGTCCTTGTCTTTGTCCGTGAAC
IFIT1	Sense: AATTAGCCACAGGTCATT	144	55
	Anti-sense: ATTCCATACACAACACTCT
ASHA2	Sense: AATCTCCTGCTATATTAGAAG	126	55
	Anti-sense: AGTTCCTACATCTCCATT
PAK1	Sense: CACTTCCTATTACTCCAACT	113	59
	Anti-sense: CTTCTTCTTCTGCTTCTCA
ST6GAL1	Sense: GACATCATTCAAGAAATCTC	164	59
	Anti-sense: AAGAACTTCTGGTAGTAGTA
RPL32 a	Sense: CGGAAGTTTCTGGTACACAATGTAA	94	55-65
	Anti-sense: TGGAAGAGACGTTGTGAGCAA

^a The transcript levels of RPL13 were used for sample normalization.