Table 2.
Strains and plasmids used in this study
| Plasmid or strain | Relevant characteristics | Source or reference |
|---|---|---|
| Plasmids |
|
|
| PUG6 |
Plasmid with loxP-kanMX-loxP disruption cassette |
[23] |
| pSP-G1 |
2μ-based URA3 plasmid with TEF1/PGK1 promoter |
[25] |
| pXI-5 |
Integrative USER vector, with reusable URA3 Marker |
[24] |
| pSH47 |
Centromeric plasmid, URA3, PGAL1-Cre-TCYC1 |
[23] |
| pXI-5-PtFPS1 |
pXI-5 with TEF1/PGK1 promoter and PtFPS1 gene |
This study |
| pXI-5-PtFPS2 |
pXI-5 with TEF1/PGK1 promoter and PtFPS2 gene |
This study |
| pXI-5-PtSTL1 |
pXI-5 with TEF1/PGK1 promoter and PtSTL1 gene |
This study |
| pXI-5-PtSTL2 |
pXI-5 with TEF1/PGK1 promoter and PtSTL2 gene |
This study |
| pXI-5-ScFPS1 |
pXI-5 with TEF1/PGK1 promoter and ScFPS1 gene |
This study |
| pXI-5- ScSTL1 |
pXI-5 with TEF1/PGK1 promoter and ScSTL1 gene |
This study |
|
S.cerevisiae Strains |
|
|
| CEN.PK 113-5D |
MATaMAL2-8cSUC2 ura3-52 |
Peter Kötter |
|
stl1Δ |
CEN.PK 113-5D Stl1Δ (490,1279 )::loxP |
This study |
| PtFPS1_XY1 |
stl1Δ with plasmid pXI-5-PtFPS1 |
This study |
| PtFPS2_XY2 |
stl1Δ with plasmid pXI-5-PtFPS2 |
This study |
| PtSTL1_XY3 |
stl1Δ with plasmid pXI-5-PtSTL1 |
This study |
| PtSTL2_XY4 |
stl1Δ with plasmid pXI-5-PtSTL2 |
This study |
| ScFPS1_XY5 |
stl1Δ with plasmid pXI-5-ScFPS1 |
This study |
| ScSTL1_XY6 | stl1Δ with plasmid pXI-5- ScSTL1 | This study |