Table 1.

Coverage summary of mTOR pathway candidate genes sequenced by next generation sequencing

Gene	Chromosome	Exons screened	Targeted exon bases	High coverage basesa	Average coverage per pool	Average coverage per probandb	Average coverage per parentb
TSC1	9	Exons 3-23	3495	3495	52804.6	1760.2	880.1
TSC2	16	Exons 1-41	5355	5355	79732.3	2657.7	1328.9
RHEB	7	Exons 2-8	555	503	16050.2	535	267.5
MYCBP2 (Pam)	13	Exons 1-83	13923	13923	21906.8	730.2	365.1
FBXO45	3	Exons 1-3	861	858	33853.7	1128.5	564.2

^aindividual bases for which at least 3000x average coverage was obtained.

^bstatistics reflect coverage per proband/parent sample, not per chromosome.