Table 1.
A/Description of primary baits
| Protein name | Protein symbol and LGMD form | Bait domain coordinates (aa)* | Bait domain description |
|---|---|---|---|
| calpain 3 |
CAPN3 (LGMD2A) |
T417-S643 |
C2-like domain + exons15-16 |
| |
M1-A822 |
full length protein with the C129S mutation in the autocatalytic site |
|
| dysferlin |
DYSF (LGMD2B) |
L2-I485 |
N-terminal DYSF regions containing the first three C2 domains |
| |
Q851-D1200 |
central DYSF domain |
|
| |
I1145-L2026 |
C-terminal DYSF domain containing the last four C2 domains and excluding the transmembrane span |
|
| γ- sarcoglycan |
SGCG (LGMD2C) |
M1-L36 |
cytoplasmic domain |
| α-sarcoglycan |
SGCA (LGMD2D) |
M313-H388 |
cytoplasmic domain |
| β-sarcoglycan |
SGCB (LGMD2E) |
E10-A65 |
cytoplasmic domain |
| δ-sarcoglycan |
SGCD (LGMD2F) |
E1-Y36 |
cytoplasmic domain |
| titin-cap (telethonin) |
TCAP (LGMD2G) |
M1-G167 |
full length protein |
| tripartite motif-containing 32 |
TRIM32 (LGMD2H) |
L66-P654 |
full length protein minus the RING domain |
| titin |
TTN (LGMD2J) |
V97-K469 |
exons 4-8 from the Z-Disc region |
| I741-G948 |
exons 14-17 from the Z-Disc region |
||
| R2120-L2564 |
exons 28-33 from the Z-Disc region |
||
| A8831-E9158 |
exons 108-114 from the N2A-PEVK region |
||
|
T32840-I33423 |
exons 358-363 from the Mline region |
||
| ankyrin repeat domain 1 |
ANKRD1 |
M1-F319 |
full length protein |
| ezrin |
EZR |
M1-S536 |
full length protein minus the actin-binding C-terminal domain |
| F-box protein 32 (MAFbx) |
FBXO32 |
M1-F356 |
full length protein |
| tripartite motif-containing 63 (MuRF1) | TRIM63 | M1-Q354 | full length protein |
* amino acid coordinates refer to the translated products of the nucleotide sequences indicated in Additional file 2: Table S2.