Figure 8. The role of HXK1 in sugar-induced miR156 repression.
(A) Expression of miR156 in the 5-day-old wild type (ecotype Ler) and gin2-1 mutant. The expression level of miR156 in Ler was set to 1. (B) Time course analyses of miR156 in the gin2-1 mutant. (C and D) Expression of pri-MIR156A (C) and pri-MIR156C (D) in response to glucose in the wild type (ecotype Ler) and gin2-1 mutant. Five-day-old seedlings in 1/2 Murashige and Skoog (MS) liquid media were treated with 50 mM glucose (Glc) or mannitol (Man) for 6 hr. The expression level in Ler at 0 h was set to 1. (E) Expression of pri-MIR156C in Ler and gin2-1. Five-day-old seedlings in 1/2 MS liquid media were pre-treated with actinomycin-D (ActD) for 12 hr and then treated with 50 mM glucose or mannitol. The expression level of pri-MIR156C in Ler at 0 hr was set to 1. Sugar treatment was started at Zeitgeber time 12.