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Effects of girinimbine on the morphological changes of A549 cells after 24 h treatment. Apoptosis was monitored by phase contrast ((a) control, (b) treatment 9 μM, and (c) treatment 19 μM) and fluorescence microscopy ((d) control, (e) treatment 9 μM, and (f) treatment 19 μM). Arrows indicate the cell shrinkage, ruffling, and blebbing of cell membrane while arrowheads indicate the annexin V binding to phosphatidylserine. Magnification 20X.
