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. Author manuscript; available in PMC: 2013 Mar 28.
Published in final edited form as: Nature. 2011 Mar 30;472(7342):226–229. doi: 10.1038/nature09873

Figure 4. ThT enhancement of lifespan depends on HSF-1 and SKN-1 transcription factors but not on DAF-16.

Figure 4

a–c, Effect of 50 μM ThT and 100 μM curcumin on Kaplan–Meier survival curves of synchronously ageing PS3551 [hsf-1(sy441)I] (a), CF1038 [daf-16(mu86)I] (b) and DA465 [eat-2(ad465)II] (c) lifespan. d, Effect of reducing SKN-1 by skn-1 RNAi on the increase in lifespan elicited by ThT. Plots are representative of three independent experiments. e, ThT and curcumin (Cur) treatment does not result in DAF-16::GFP relocalization as compared to control strain [daf-16::daf-16-gfp + rol-6] (upper left). Control strain under heat shock (HS) produces a clear relocalization of DAF-16::GFP to the nuclei of intestinal cells.