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. 2013 Mar 18;14:189. doi: 10.1186/1471-2164-14-189

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Copyright ©2013 Paul et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The analysis of the orthologous species. (a) The phylogenetic relation of the plant species analysed via OrthoMCL (Additional files 2 and 4) is given. (b) Correlation of the number of protein sequences to the number of orthologues for all 14 plant species discussed (c) The according orthologues in S. lycopersicum, S. cerevisiae and A. thaliana (left scatterplot) and S. lycopersicum to A. thaliana (right scatterplot) have been analysed with respect to amino acid number (protein length in amino acids). The line in the right scatterplot represent the least square fit analysis to y=a*x with a=0.992. (d) Domain architecture of cpSecA2 orthologues from A.thaliana and S. lycopersicum. AT1G21650 corresponds to Solyc11g005020 whereas AT1G21651 corresponds to the combination of Solyc11g005040 and Solyc11g005030. (e) RT-PCR results confirming that Solyc11g005040 and Solyc11g005030 is ‘one gene’; lanes 1 to 4: cDNA, genomic DNA, no-RT control (without reverse transcriptase), negative control (water), respectively.