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. 2013 Mar 19;7:149–159. doi: 10.2147/DDDT.S42390

Table 1.

Primer sequences and annealing temperature of the genes in RT-PCR

Gene Gene sequences Product size (bp) Annealing temperature (°C)
DDB1 Forward primer 5′-ATCATCCGGAATGGAATTGGAA-3′
Forward primer 5′-TCAGACCGCAGTGGCCATAA-3′
83 60
RAD51 Forward primer 5′-TGGGAACTGCAACTCATCTGG-3′
Forward primer 5′-GCGCTCCTCTCTCCAGCAG-3′
157 61
XRCC5 Forward primer 5′-GCCATATCAGTGAACCTTTAGAGAC-3′
Forward primer 5′-GGAACTGGAACTCAAGGCAAG-3′
268 59
PCNA Forward primer 5′-CTGTAGCGGCGTTGT-3′
Reverse primer 5′-ACTTTCTCCTGGTTTGG-3′
362 52
ABCC4 Forward primer 5′-GCTCACTGGATTGTCTTCATTTTC-3′
Reverse primer 5′-CTCGGTTACATTTCCTCCTCCAT-3′
147 59
β-actin Forward primer 5′-CCTGAGGCTCTTTTCCAGCC-3′
Reverse primer 5′-TAGAGGTCTTTACGGATGTCAACGT-3′
110 60

Notes: Five genes, including DDB1, RAD51, XRCC5, PCNA, and ABCC4 were confirmed to their expression, in microarray analysis using RT- PCR, and were significantly expressed after RPM treatment in PC-2 cells. The primer pairs and the predicted sizes of the amplified PCR products and the annealing temperatures of PCR were listed. β-actin is the housekeeping gene for RT- PCR analysis. The primer sequences were designed with Primer Premier 5.0 software (Premier Biosoft, Palo Alto, CA, USA).

Abbreviations: PCR, polymerase chain reaction; RPM, rapamycin; RT, reverse transcription.