Table 1.

Primer sequences and annealing temperature of the genes in RT-PCR

Gene	Gene sequences	Product size (bp)	Annealing temperature (°C)
DDB1	Forward primer 5′-ATCATCCGGAATGGAATTGGAA-3′ Forward primer 5′-TCAGACCGCAGTGGCCATAA-3′	83	60
RAD51	Forward primer 5′-TGGGAACTGCAACTCATCTGG-3′ Forward primer 5′-GCGCTCCTCTCTCCAGCAG-3′	157	61
XRCC5	Forward primer 5′-GCCATATCAGTGAACCTTTAGAGAC-3′ Forward primer 5′-GGAACTGGAACTCAAGGCAAG-3′	268	59
PCNA	Forward primer 5′-CTGTAGCGGCGTTGT-3′ Reverse primer 5′-ACTTTCTCCTGGTTTGG-3′	362	52
ABCC4	Forward primer 5′-GCTCACTGGATTGTCTTCATTTTC-3′ Reverse primer 5′-CTCGGTTACATTTCCTCCTCCAT-3′	147	59
β-actin	Forward primer 5′-CCTGAGGCTCTTTTCCAGCC-3′ Reverse primer 5′-TAGAGGTCTTTACGGATGTCAACGT-3′	110	60

Notes: Five genes, including DDB1, RAD51, XRCC5, PCNA, and ABCC4 were confirmed to their expression, in microarray analysis using RT- PCR, and were significantly expressed after RPM treatment in PC-2 cells. The primer pairs and the predicted sizes of the amplified PCR products and the annealing temperatures of PCR were listed. β-actin is the housekeeping gene for RT- PCR analysis. The primer sequences were designed with Primer Premier 5.0 software (Premier Biosoft, Palo Alto, CA, USA).

Abbreviations: PCR, polymerase chain reaction; RPM, rapamycin; RT, reverse transcription.