Table 2.
The DNA damage repair and transcription genes differentially expressed in PC-2 cells induced by RPM
| Genbank ID | Gene description | Ratio (Cy5/Cy3) |
|---|---|---|
| NM_016931 | Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4) | 0.16 |
| NM-001923 | Damage-specific DNA binding protein (DDB1) | 0.20 |
| NM_018682 | Myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila) (MLL5) | 0.21 |
| NM_013974 | Dimethylarginine dimethylaminohydrolase 2 (DDAH2) | 0.23 |
| NM_001675 | Activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4) | 0.27 |
| NM_003489 | Nuclear receptor interacting protein 1 (NRIP1) | 0.28 |
| BC049823 | Ribosomal protein L22-like 1 (RPL22) | 0.30 |
| NM_175623 | RAB3A interacting protein (rabin3) (RAB3IP), transcript variant alpha 2 | 0.32 |
| BC022217 | Chromosome 6 open reading frame 85(C6ORF85) | 0.33 |
| NM-005431 | X-ray repair complementing defective repair in Chinese hamster cells 2 (XRCC2) | 0.33 |
| NM_000689 | Aldehyde dehydrogenase 1 family, member A1 (ALDH1A1) | 0.36 |
| NM_002876 | RAD51 homolog C (S. cerevisiae) (RAD51C), transcript variant 2 | 0.37 |
| NM_001008712 | RNA binding protein with multiple splicing (RBPMS), transcript variant 3 | 0.38 |
| NM_139207 | Nucleosome assembly protein 1-like 1 (NAP1L1) | 0.39 |
| NM_000075 | Cyclin-dependent kinase 4 (CDK4) | 0.40 |
| NM_053277 | Chloride intracellular channel 6 (CLIC6) | 0.40 |
| NM-021141 | X-ray repair complementing defective repair in hamster cells 5 (XRCC5) | 0.41 |
| NM_001380 | Dedicator of cytokinesis 1 (DOCK1) | 0.42 |
| NM_014515 | CCR4-NOT transcription complex, subunit 2 (CNOT2) | 0.42 |
| NM_005655 | Transforming growth factor-beta inducible early growth response (TGFBI EGR) | 0.42 |
| NM-003852 | Transcriptional intermediary factor 1 (TIF 1) | 0.43 |
| NM_152721 | Docking protein 6 (DOK6) | 0.46 |
| NM_139207 | Nucleosome assembly protein 1-like 1 (NAP1L1) | 0.46 |
| NM_006312 | Nuclear receptor corepressor 2 (NCOR2) | 0.48 |
| NM_000538 | Regulatory factor X-associated protein (RFXAP) | 0.49 |
| NM_016545 | Immediate early response 5 (IER5) | 2.35 |
| NM-002129 | High-mobility group box 2 (HMGB2) | 2.41 |
| BC005107 | Chromosome 21 open reading frame 105, mRNA (C21ORF10) | 2.43 |
| NM-006608 | Putative homeodomain transcription factor 1 (PHTF 1) | 2.57 |
| NM_178820 | F-box protein 27 (FBXO27) | 2.61 |
| NM_000927 | ATP-binding cassette, subfamily B (MDR/TAP), member 1 (ABCB1) | 2.73 |
| NM_002592 | Proliferating cell nuclear antigen (PCNA) | 2.85 |
| NM_001924 | Growth arrest and DNA-damage-inducible, alpha (GADD45A) | 2.91 |
| NM_012153 | Ets homologous factor (EHF) | 3.13 |
| NM_005845 | ATP-binding cassette, subfamily C (CFTR/MRP), member 4 (ABCC4) | 3.24 |
| NM_199242 | Unc-13 homolog D (C. elegans) (UNC13D) | 3.24 |
| NM_172366 | F-box protein 16 (FBXO16) | 3.28 |
| NM_000107 | Damage-specific DNA binding protein 2 (DDB2) | 3.35 |
| NM_000392 | ATP-binding cassette, subfamily C (CFTR/MRP), member 2 (ABCC2) | 3.38 |
| NM_002439 | MutS homolog 3 (MSH3) | 3.42 |
| NM_020165 | RAD18 homolog (S. cerevisiae) (RAD18) | 3.45 |
| NM_006332 | Interferon, gamma-inducible protein 30 (IFI30) | 3.57 |
| NM_018965 | Triggering receptor expressed on myeloid cells 2 (TREM2) | 4.35 |
| BC009507 | Interferon, alpha-inducible protein (clone IFI-15K) | 6.59 |
Notes: Total RNA from PC-2 cells treated with 10 nmol/L RPM for 48 hours was extracted for microarray analysis. Representative genes were significantly upregulated or downregulated by RPM in PC-2 cells.
Abbreviation: RPM, rapamycin.