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. 2013 Apr;23(4):385–391. doi: 10.1089/thy.2012.0644

FIG. 3.

FIG. 3.

(A) qRT-PCR analysis for thyroid-specific gene activity and Foxe1 expression in Pax8+, NKx2-1+, and Pax8+/NKx2-1+ ES cell lines. Inset: The quantitative reverse-transcription polymerase chain reaction (qRT-PCR) data for rat thyroid (FRTL5) cells. Relative expression of each transcript is presented as fold change compared to ES cells (mean±SEM, n=3), and represent one of three separate experiments. (B) RT-PCR analysis for thyroid-specific gene expression in untreated Pax8+/NKx2-1+ embryoid bodies (EB) cells. The gel images represent one of three separate experiments.