FIG. 4.

(A) qRT-PCR analysis of NIS, TSHR, Pax8, Tg, and TPO genes in EB cells and in EB cells treated for five days with activin A. Although there was significant expression of NIS, TSHR, and Pax8 after activin treatment, there was no induction of Tg or TPO in these cells. (B) qRT-PCR analysis for TSHR and endoderm markers (AFP, Sox17, and Fox2) in EB cells formed from activin A treated and untreated Pax8+/NKx2-1+ ES cells. Data were expressed as mean±SEM, and represent one of three separate experiments. All the data were significantly different from –LIF cells (p<0.01). LIF, leukemia inhibitory factor; AA, activin A.