Table 1.
Forward (F) and reverse (R) primers used for quantitative RT-PCR.
| Gene | Primer sequence | Product length | GenBank No. |
|---|---|---|---|
| Collagen type I (COL1A2) | F: 5′-CGGGTCTTGCTGGTCATCAT-3′ R: 5′-TGCACCAGGCTGTCCAATG-3′ |
125 | NM_174520.2 |
| Collagen type II (COL2A1) | F: 5′-AGTGGAAGAGCGGAGACTACTG-3′ R: 5′-GTTGGGAGCCAGGTTGTCAT-3′ |
233 | NM_001001135.2 |
| Collagen type X (COL10A1) | F: 5′-CTGAGCGATACCAAACACC-3′ R: 5′-CCTCTCAGTGATACACCTTTA-3′ |
106 | NM_174634.1 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
F: 5′-GAGTCCACTGGGGTCTTCACT-3′ R: 5′-GCGTGGACAGTGGTCATAAGTC-3′ |
251 | NM_001034034.1 |