Table 1.

ELISA Results from Mouse Serum.

Marker	Control	PanIN1	P Value	PanIN2/3	P Value (vs Con)	P Value (vs PanIN1)	Invasive CA	P Value (vs Con)	P Value (vs PanIN1)
CXCL16	0.65 ng/ml (N = 9)	0.69 ng/ml (N = 5)	.753*	1.45 ng/ml (N = 8)	.0004	.0152	6.96 ng/ml (N = 6)	.0001	.0001
TIMP1	2.9 ng/ml (N = 6)	4.6 ng/ml (N = 6)	.035	6.6 ng/ml (N = 10)	.0021	.0805*	35.8 ng/ml (N = 6)	.0123	.0162
LCN2	165 ng/ml (N = 17)	337 ng/ml (N = 16)	.0008	1937 ng/ml (N = 10)	.0001	.0001	4249 ng/ml (N = 9)	<.0001	.0001

ELISA tests were performed according to the recommendations by the manufacturer for CXCL16, TIMP1, and LCN2/NGAL (R&D Systems). The ELISA plate was measured on an Emax plate reader (Molecular Devices, Munich Germany) and then analyzed with SOFTmax Pro (Version 3-0) software.

To determine whether our assays show differences between healthy (wild-type) and affected (transgenic) animals, we evaluated serum protein levels from each of the ELISAs using an unpaired t test.

Values of P < .05 were considered to be statistically significant.

^*Not statistically significant.