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. 2013 Apr 1;6(2):216–225. doi: 10.1593/tlo.13133

Figure 4.

Figure 4

Disrupting the CXCR2 macromolecular complex inhibits PDAC cell proliferation. (A) HPAC, (B) Colo357, and (C) HPDE cell proliferation in response of CXCL1/CXCL5/CXCL8 (100 ng/ml) was assessed and quantified by MTT assay and expressed as relative to the initial time point (0 hour). Cell proliferation in response to indicated chemokines (CXCL5 or CXCL8; 100 ng/ml) in Colo357 and HPAC cells, which were transfected with plasmids expressing CXCR2 C-tail WT or ΔTTL (D, E) or pre-delivered with CXCR2 C-tail-specific peptide WT or ΔTTL (F, G). (H) Pairwise binding between GST-NHERF1 and various biotin-conjugated CXCR2 C-tail-specific peptide (containing last 13 amino acids) WT, ΔTTL, or AAA. The complex was pulled down by streptavidin agarose and immunoblotted with anti-NHERF1 antibody. Columns/dots, means of quintuplicates; bars, SEM; *P < .05. NT, cells not transfected or pre-delivered with peptides.