A, Multiple sequence alignment of mouse, rat, and human Vegfa promoter regions (from −582 to −611 bps) containing ERRγ binding site (box; 5′-TGACCT-3′ and 3′-ACTGGAA-5′). Highly conserved nucleotides are shaded. A consensus sequence is also shown at the bottom. Closed arrows show the locations of polymerase chain reaction primers flanking the conserved site used in chromatin immunoprecipitation polymerase chain reaction. Open arrow shows transcription start site (+1). The map is not drawn to scale. B, Luciferase reporter assay showing activation of the Vegfa promoter by ERRγ in transfected 293T cells subjected to normoxia or hypoxia for 24 hours. C, Chromatin immunoprecipitation polymerase chain reaction demonstrating ERRγ occupancy at the conserved binding site in promoter region of the endogenous Vegfa gene in differentiated C2C12 cells overexpressing the receptor under normoxia and hypoxia.