Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 28;9(3):e1003004. doi: 10.1371/journal.pcbi.1003004

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Mukhopadhyay et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A) The concentration of bound ZAP-70 as a function of the relative concentration of active kinase (E) to phosphatase (F). Shown are the wild-type -chain (123), and additional constructs where all ITAMs are identical (222), switched (321), or where phosphorylation is no longer sequential (123 Random). The Hill numbers (inset) reveal that ultrasensitivity is decreased if ZAP-70 does not exhibit differential affinity (222), if the affinity decreases as the is sequentially phosphorylated (321), or if phosphorylation is no longer sequential. B) Heat map of Hill numbers as a function of the ZAP-70 unbinding rate for ITAM 1 (Z) and ITAM 3 (Z), where the unbinding rate for ITAM 2 is fixed at 1 s⁻¹. The calculation is performed under sequential phosphorylation. Maximum sensitivity is found in the top left of the heat map, where ZAP-70 binds with the largest affinity to ITAM 1 and with lowest affinity to ITAM 3. The heat map is repeated using an alternate measure of sensitivity in Fig. S3. Note that the heat map colour scheme in panel B is not related to the colour scheme in panel A.

Inline graphic — A) The concentration of bound ZAP-70 as a function of the relative concentration of active kinase (E) to phosphatase (F). Shown are the wild-type -chain (123), and additional constructs where all ITAMs are identical (222), switched (321), or where phosphorylation is no longer sequential (123 Random). The Hill numbers (inset) reveal that ultrasensitivity is decreased if ZAP-70 does not exhibit differential affinity (222), if the affinity decreases as the is sequentially phosphorylated (321), or if phosphorylation is no longer sequential. B) Heat map of Hill numbers as a function of the ZAP-70 unbinding rate for ITAM 1 (Z) and ITAM 3 (Z), where the unbinding rate for ITAM 2 is fixed at 1 s⁻¹. The calculation is performed under sequential phosphorylation. Maximum sensitivity is found in the top left of the heat map, where ZAP-70 binds with the largest affinity to ITAM 1 and with lowest affinity to ITAM 3. The heat map is repeated using an alternate measure of sensitivity in Fig. S3. Note that the heat map colour scheme in panel B is not related to the colour scheme in panel A.