Figure 9.

Interleukin-21 (IL-21) depletion at priming significantly alters the number FoxP3⁺, RORγt⁺ (related orphan receptor-γt⁺), and T-bet⁺ CD4 T cells 28 days post respiratory syncytial virus challenge. Mice were treated as in Figure 2. Twenty-eight days post challenge, spleen cells from both the groups plus naive mice were harvested and processed. CD4 T cells were stained for (a) FoxP3, (b) RORγt, or (c) T-bet according to the manufacturer's instructions. The percentage of CD4 T cells expressing each transcription factor was determined by flow cytometry and is shown in each dotplot. Grouped data for (d) percentage and (e) total number are also shown. The graph is representative of two independent experiments of five mice per group. Student's t-test result; **P<0.01, ***P<0.001.