Figure 3. ESR spectra of B-FABP mutants D17R1, E18R1, M20R1 and K21R1, in the absence (black) and in the presence of LPC (gray) or LPG (light gray) micelles in 20 mM phosphate buffer (pH 8.0).

Except for mutant M20R1, the mutants presented decreased mobility upon addition of the membrane mimetic. The arrow denotes the more immobilized population in D17R1 spectrum, which showed up in the presence of the micelles.