Figure 2. Iron absorption genes are regulated in copper deficiency.
(A) Relative mRNA levels of Cybrd1, Slc40a1 and Slc11a2 +IRE mRNA in the duodenum of mice on control or CuD diets. mRNA levels of control mice were adjusted to 1. (B) Immunoblots of Dmt1, Fpn and Dcytb (membrane fraction). β-actin was used as a loading control. Changes in protein levels were quantified using ImageJ software. 1-way ANOVA with Newman-Keuls posthoc testing. Data are presented as means±SEM: CTR (n = 6), CuD (n = 6), CuD+CuSO4 (n = 4). Means without a common letter differ (p<.05).