Figure 5. Mus81-mediated DSBs in 31FR-31NR cells. (A) Western blotting results for Mus81, γ-H2AX and actin in 0FR and 31FR-31NR cells derived from HepG2 cell line. Cell extracts were prepared at 24 h after transfection with either control siRNA (Cont.) or Mus81 siRNA. The amounts of γ-H2AX were normalized by corresponding actin level. The values are expressed relative to the control value of 0FR cells with control siRNA. (B) Cyclin D1 (green) and γ-H2AX (red) in 0FR cells and 31FR-31NR cells of HeLa are shown. Immunofluoresence were performed 48 h after transfection with control siRNA or Mus81 siRNA. Double-staining cells with cyclin D1 and γ-H2AX are indicated by arrows.