Figure 3. Cellular phenotypes of human cen3tel skin cells from a centenarian female 37 (A), 100 (B), 166 (C) and 1014 (D) generations after transfection with overexpressed telomerase. The culture had a fibroblastic cellular phenotype at PD37 (A) and a less defined fibroblastic phenotype at generation PD100 (B). At generation PD166, the culture had acquired a polymorphic cellular phenotype typical of cancer cells and simultaneously became tumorigenic and immortal (C). This new polymorphic phenotype was unchanged at PD1014 (D).