Figure 4. TD-60 knockdown suppresses proliferation and mitotic markers. (A) Quantitative assay of HeLa cells following siRNA transfection shows the cell population ceases proliferation and remains blocked for 3 days. Viable cells were counted relative to time 0 (time of transfection). The control was a sham transfection. (B) Immunoblot analysis of Cyclin B1 and Cyclin A of TD-60 siRNA-transfected cells treated with nocodazole (0.04 μg/ml) from 24 to 48 h or 48 h to 72 h (left), and of Cdk1 phospho-substrates after nocodazole treatment from 48 h to 72 h (right). The blots confirm absence of mitotic markers and Cdk1 phospho-substrates after TD-60 depletion.